FabRICATOR® Z (IdeZ) Smart Enzymes

FabRICATOR Z is an enzyme for preparation of F(ab’)2 from mouse subclasses IgG2a and IgG3. FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, generating intact F(ab’)2 and Fc fragments. Most mouse IgG2a and IgG3 are digested by FabRICATOR Z within 2 hours, although the enzyme:antibody ratio and/or digestion time may need to be optimized for certain antibodies. For digestion of human IgGs, FabRICATOR® is recommended.

FabRICATOR Z digests IgG at a specific site and there is no risk of overdigestion if the incubation time is prolonged. FabRICATOR Z digests IgG at mild conditions, thus preserving the immunoreactivity. FabRICATOR Z digests IgG in sodium phosphate buffer with pH ranging from 6.0-8.0, but is most active at pH 6.5 and at low NaCl concentrations.

FabRICATOR Z is derived from S. equi ssp. zooepidemicus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 36 442 Da.